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Reactions were performed using a 20-L mixed system containing 7.2 L of sterile distilled water, 10 L of qPCR SYBR Green Master Mix (Yeasen Biotechnology), 0.8 L of each gene-specific primer, and 2 L of the cDNA template. Tokuyama is 100% committed to Innovating Tomorrows Dentistry, Today. Each sample was analyzed in three replicates. Its universal formulation works with all bonding substratesincluding dentin, enamel, porcelain, metal, composite, and zirconiaand it has higher bond strengths across more indirect restorative materials than leading competitors 8, Full PDF Package Download Full PDF Package. Outdoor ageing performance of elastotec ceramic lagging Ceramic lagging is widely used in applications that require increased levels of grip and long service life. Rating: 0%. I don't scan in my practice my assist does. This Paper. Dent Today. PDF Pack. Radiolucency under an existing restoration can lead to misdiagnosis and potentially invasive overtreatment. al. 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